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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two added splice variants were described but detailed nucleotide sequence details is not really yet offered.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically is made up of the epitope recognized through the antibody.
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to be certain the very best success are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to guarantee the best possible final results are...
Antibodies certain to the blocking peptide not bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is specific.
Goal Info Cyclin E belongs on the hugely conserved cyclin relatives, whose customers are characterised by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E types a complex get more info with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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